What is a purge tube for blood collection?

If a “butterfly needle” is being used to collect blood, a “purge” tube (vacutainer red or marble top)should be used to ensure that the tubing is filled with blood prior to the QuantiFERON®-TB Gold tubes being used.

What are the tubes used in phlebotomy?


Green Sodium heparin(100 USP Units) Ammonia, Lactate, HLA Typing
Tan K2 EDTA Lead levels
Yellow ACD Solution A consists of trisodium citrate, citric acid and dextrose DNA Studies, HIV Cultures
Pink (K2)EDTA Blood type & Screen, Compatibility Study, Direct Coombs HIV Viral Load

What is a discard tube and when do you use it?

The discard tube must be used to prime the tubing of the collection set, which will assure maintenance of the proper anticoagulant/blood ratio in the first tube filled. The discard tube should be a non-additive or a coagulation tube, and need not be completely filled.

What blood tube colors are for which test?

Blood Collection Tubes

Tube cap color Additive
Red or gold (mottled or “tiger” top used with some tubes) Serum tube with or without clot activator or gel
Green Sodium or lithium heparin with or without gel
Lavender or pink Potassium EDTA
Gray Sodium fluoride, and sodium or potassium oxalate

What tubes can be used as discard tube?

discard tube prior to coagulation collection. Another citrate (blue top) tube should be used as the discard tube. 3. Serum – Inside walls of tube are coated with a clot activator to promote coagulation.

What is EDTA tube used for?

It’s a tube coated with spray-dried K2EDTA on its inner wall. EDTA acts as an anticoagulant, binding the calcium ions and interrupting the clotting of the blood sample. EDTA is used for most hematology procedures, assessing complete blood count, preparing EDTA plasma, whole blood collection and bone marrow specimens.

How many phlebotomy tubes are there?

seven tubes
In phlebotomy, this term has special meaning as it is the order in which certain tubes can be used. There are seven tubes which make up the order of draw. They are listed by chemical additives that have been introduced to the tube by the manufacturer to perform a specific function when mixed with the blood.

What do different color blood vials mean?

The tests each bottle is used for are the same: the purple one is for cell count, the yellow one is for electrolytes, albumin and LDH, the grey one is for glucose, and blood culture bottles can be used for fluid cultures.

What is the order of draw?

The “Order of Draw” is designed to eliminate the possibility of cross contamination that may result in erroneous results. It is based on CLSI Procedures for Collection of Diagnostic Blood Specimens by Venipuncture; Approved Standard Sixth Edition, October 2007.

What order should you draw in when drawing a TB QuantiFERON tube?

It is recommended to draw in the order grey, green, yellow, purple. As the 1 ml tubes draw relatively slowly, keep the tube on the needle for 2-3 seconds once the tube appears to have completed filling.

What is the GREY tube used for in phlebotomy?

Grey-top tube (potassium oxalate/sodium fluoride) This tube contains potassium oxalate as an anticoagulant and sodium fluoride as a preservative – used to preserve glucose in whole blood and for some special chemistry tests.