What is Holliday junction in recombination?

Holliday junction, cross-shaped structure that forms during the process of genetic recombination, when two double-stranded DNA molecules become separated into four strands in order to exchange segments of genetic information.

Which enzyme is important in the resolution of a Holliday junction?

Holliday junctions and their processing The most widespread is by the action of junction-resolving enzymes, nucleases that are selective for branched DNA.

What does Holliday junction do?

The Holliday junction is a key intermediate in homologous recombination, a biological process that increases genetic diversity by shifting genes between two chromosomes, as well as site-specific recombination events involving integrases. They are additionally involved in repair of double-strand breaks.

What are Holliday intermediates?

Holliday intermediate An intermediate structure in genetic recombination, proposed by Robin Holliday in 1964, in which two double-stranded homologous DNA molecules are joined by means of a reciprocal crossover involving one DNA strand of each molecule.

Which proteins function in the resolution of Holliday junctions?

RuvC is a dimeric protein that promotes HJ resolution by introducing a pair of symmetrically related nicks in two strands that lie diametrically opposed across the junction point.

How does Holliday model of recombination repair DNA breaks?

In the original Holliday model for homologous recombination, single-strand breaks occur at the same point on one strand of each parental DNA. Free ends of each broken strand then migrate across to the other DNA helix.

What does the Spo11 protein do during the recombination?

Spo11 is a protein that in humans is encoded by the SPO11 gene. Spo11, in a complex with mTopVIB, creates double strand breaks to initiate meiotic recombination. Its active site contains a tyrosine which ligates and dissociates with DNA to promote break formation.