Which siderophore is produced by Pseudomonas fluorescens?
Pyoverdines are siderophores synthesized by fluorescent Pseudomonas spp. Under iron-limiting conditions, these high-affinity ferric iron chelators are excreted by bacteria in the soil to acquire iron. Pyoverdines produced by beneficial Pseudomonas spp.
Which siderophore is produced by Pseudomonas aeruginosa?
Pseudomonas aeruginosa produces siderophores, pyoverdin and pyochelin, for high-affinity iron uptake. To investigate their contribution to P. aeruginosa infections, we constructed allelic exchange mutants from strain PAO1 which were deficient in producing one or both of the siderophores.
What is the fluorescent pigment produced by Pseudomonas fluorescens?
Both its biosynthesis and its chemical properties (formation of a stable Fe3+ complex) suggest that the fluorescent pigment is a desferrisiderophore.
How does CAS assay work?
The chrome azurol S (CAS) assay is a universal colorimetric method that detects siderophores independent of their structure. In this assay, siderophores scavenge iron from an Fe-CAS-hexadecyltrimethylammonium bromide complex, and subsequent release of the CAS dye results in a color change from blue to orange.
What type of siderophore is pyoverdine?
Also, pyoverdine, a complex mixed-type siderophore that is expressed by fluorescent Pseudomonas spp., contains both hydroxamate and catecholate groups (Fig. 1) (Schalk, Hannauer, & Braud, 2011).
What is the use of Pseudomonas fluorescens?
Many strains of Pseudomonas fluorescens are known to enhance plant growth promotion and reduce severity of various diseases. The efficacy of bacterial antagonists in controlling fungal diseases was often better as alone, and sometimes in combination with fungicides.
Does Pseudomonas fluorescens produce polyhydroxyalkanoates?
Pseudomonas fluorescens KLR101 was found to be capable of producing polyhydroxyalkanoate (PHA) using various sugars and fatty acids with carbon numbers ranging from 2 to 6.
What is the Pseudomonas fluorescens group?
Pseudomonas fluorescens is a common Gram-negative, rod-shaped bacterium. It belongs to the Pseudomonas genus; 16S rRNA analysis as well as phylogenomic analysis has placed P. fluorescens in the P. fluorescens group within the genus, to which it lends its name.
What is a siderophore cephalosporin?
Cefiderocol is a siderophore cephalosporin antibiotic that has recently been developed to combat a variety of bacterial pathogens, including β-lactam- and carbapenem-resistant organisms.